Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses
Identifieur interne : 000221 ( France/Analysis ); précédent : 000220; suivant : 000222Cytomegalovirus (CMV) glycoprotein B genotype and CMV DNA load in the amniotic fluid of infected fetuses
Auteurs : Olivier Picone [France] ; Jean-Marc Costa [France] ; Marianne Leruez-Ville [France] ; Pauline Ernault [France] ; Martine Olivi [France] ; Yves Ville [France]Source :
- Prenatal Diagnosis [ 0197-3851 ] ; 2004-12-15.
English descriptors
- Teeft :
- Amniotic, Asymptomatic, Asymptomatic fetuses, Barbi, Bowel, Clin microbiol, Clinical data, Congenital, Congenital cytomegalovirus infection, Congenital infection, Copyright, Cytomegalovirus, Diagn, Echogenic, Echogenic bowel, Fetal, Fetal infection, Fetus, Genotype, Gestational, Glycoprotein, Gouarin, Guerra, Human cytomegalovirus, Human cytomegalovirus glycoprotein, Hyperechogenic, Hyperechogenic bowel, Immunocompromised patients, Infection, Iugr, John wiley sons, Median, Microcephaly, Ndings, Prenat, Prenat diagn, Revello, Spearman rank test, Symptomatic fetuses, Ultrasound, Ultrasound features, Ventriculomegaly, Viral, Viral load, Viral loads.
Abstract
Objective: To assess the value of both cytomegalovirus (CMV) DNA quantification in amniotic fluid (AF) and CMV glycoprotein B (gB) genotype as prognostic factors in CMV congenital infection. Methods: Quantification of CMV DNA was performed prospectively by real‐time PCR and gB genotypes were analysed by gene sequencing analysis in the amniotic fluid of 42 fetuses infected by CMV. These were correlated with clinical data on fetal anomalies and outcome. Results: The proportion of severely symptomatic fetuses was similar in each gB genotype group. Median CMV DNA load was higher in the group of symptomatic fetuses but this difference was not significant and high and low viral loads were found in both groups. Conclusion: Neither gB genotype nor CMV DNA load in AF correlate with the severity of CMV congenital infection and these markers are unlikely to prove useful for the management of congenital infection. Copyright © 2004 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/pd.942
Affiliations:
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type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Ernault, Pauline" sort="Ernault, Pauline" uniqKey="Ernault P" first="Pauline" last="Ernault">Pauline Ernault</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Moléculaire Marcel Dassault, Hôpital Américain de Paris, Neuilly‐sur‐Seine</wicri:regionArea><wicri:noRegion>Neuilly‐sur‐Seine</wicri:noRegion><wicri:noRegion>Neuilly‐sur‐Seine</wicri:noRegion></affiliation></author><author><name sortKey="Olivi, Martine" sort="Olivi, Martine" uniqKey="Olivi M" first="Martine" last="Olivi">Martine Olivi</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Moléculaire Marcel Dassault, Hôpital Américain de Paris, Neuilly‐sur‐Seine</wicri:regionArea><wicri:noRegion>Neuilly‐sur‐Seine</wicri:noRegion><wicri:noRegion>Neuilly‐sur‐Seine</wicri:noRegion></affiliation></author><author><name sortKey="Ville, Yves" sort="Ville, Yves" uniqKey="Ville Y" first="Yves" last="Ville">Yves Ville</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Service de Gynécologie Obstétrique, Hôpital de Poissy‐St‐Germain, Poissy</wicri:regionArea><wicri:noRegion>Poissy</wicri:noRegion><wicri:noRegion>Poissy</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Correspondence address: Centre Hospitalier Intercommunal de Poissy‐St Germain, 10 rue du Champ Gaillard, 78300 Poissy</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Poissy</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Prenatal Diagnosis</title><title level="j" type="alt">PRENATAL DIAGNOSIS</title><idno type="ISSN">0197-3851</idno><idno type="eISSN">1097-0223</idno><imprint><biblScope unit="vol">24</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1001">1001</biblScope><biblScope unit="page" to="1006">1006</biblScope><biblScope unit="page-count">6</biblScope><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2004-12-15">2004-12-15</date></imprint><idno type="ISSN">0197-3851</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0197-3851</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amniotic</term><term>Asymptomatic</term><term>Asymptomatic fetuses</term><term>Barbi</term><term>Bowel</term><term>Clin microbiol</term><term>Clinical data</term><term>Congenital</term><term>Congenital cytomegalovirus infection</term><term>Congenital infection</term><term>Copyright</term><term>Cytomegalovirus</term><term>Diagn</term><term>Echogenic</term><term>Echogenic bowel</term><term>Fetal</term><term>Fetal infection</term><term>Fetus</term><term>Genotype</term><term>Gestational</term><term>Glycoprotein</term><term>Gouarin</term><term>Guerra</term><term>Human cytomegalovirus</term><term>Human cytomegalovirus glycoprotein</term><term>Hyperechogenic</term><term>Hyperechogenic bowel</term><term>Immunocompromised patients</term><term>Infection</term><term>Iugr</term><term>John wiley sons</term><term>Median</term><term>Microcephaly</term><term>Ndings</term><term>Prenat</term><term>Prenat diagn</term><term>Revello</term><term>Spearman rank test</term><term>Symptomatic fetuses</term><term>Ultrasound</term><term>Ultrasound features</term><term>Ventriculomegaly</term><term>Viral</term><term>Viral load</term><term>Viral loads</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Objective: To assess the value of both cytomegalovirus (CMV) DNA quantification in amniotic fluid (AF) and CMV glycoprotein B (gB) genotype as prognostic factors in CMV congenital infection. Methods: Quantification of CMV DNA was performed prospectively by real‐time PCR and gB genotypes were analysed by gene sequencing analysis in the amniotic fluid of 42 fetuses infected by CMV. These were correlated with clinical data on fetal anomalies and outcome. Results: The proportion of severely symptomatic fetuses was similar in each gB genotype group. Median CMV DNA load was higher in the group of symptomatic fetuses but this difference was not significant and high and low viral loads were found in both groups. Conclusion: Neither gB genotype nor CMV DNA load in AF correlate with the severity of CMV congenital infection and these markers are unlikely to prove useful for the management of congenital infection. Copyright © 2004 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li><li>Poissy</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Picone, Olivier" sort="Picone, Olivier" uniqKey="Picone O" first="Olivier" last="Picone">Olivier Picone</name></noRegion><name sortKey="Costa, Jean Arc" sort="Costa, Jean Arc" uniqKey="Costa J" first="Jean-Marc" last="Costa">Jean-Marc Costa</name><name sortKey="Ernault, Pauline" sort="Ernault, Pauline" uniqKey="Ernault P" first="Pauline" last="Ernault">Pauline Ernault</name><name sortKey="Leruez Ille, Marianne" sort="Leruez Ille, Marianne" uniqKey="Leruez Ille M" first="Marianne" last="Leruez-Ville">Marianne Leruez-Ville</name><name sortKey="Olivi, Martine" sort="Olivi, Martine" uniqKey="Olivi M" first="Martine" last="Olivi">Martine Olivi</name><name sortKey="Picone, Olivier" sort="Picone, Olivier" uniqKey="Picone O" first="Olivier" last="Picone">Olivier Picone</name><name sortKey="Ville, Yves" sort="Ville, Yves" uniqKey="Ville Y" first="Yves" last="Ville">Yves Ville</name><name sortKey="Ville, Yves" sort="Ville, Yves" uniqKey="Ville Y" first="Yves" last="Ville">Yves Ville</name><name sortKey="Ville, Yves" sort="Ville, Yves" uniqKey="Ville Y" first="Yves" last="Ville">Yves Ville</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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